| .EAR | Oracle Corporation | File audio | Java Enterprise Archive File | WebSphere Application Server | IBM |
| .EC3 | Epson US | File audio | EPSON Print CD File | EPSON Print CD | Epson US |
| .ECELP7470 | Nuera Communications | File audio | Nuera Audio File | Unknown Software | Unknown Developer |
| .EFA | Chicken Systems | File audio | Ensoniq ASR File | Chicken Systems Translator | Chicken Systems |
| .EFE | E-mu Systems | File audio | Ensoniq EPS File | Chicken Systems Ensoniq Disk Tools | Chicken Systems |
| .EFK | FMJ-Software | File audio | Ensoniq KT Instrument File | Awave Studio | FMJ-Software |
| .EFQ | Chicken Systems | File audio | Ensoniq SQ1/SQ2/KS-32 File | Chicken Systems Translator | Chicken Systems |
| .EFS | ESRI | File audio | ESRI Spatial Binary File | ESRI ArcGIS for Desktop | ESRI (Environmental Systems Research Institute) |
| .EFV | Chicken Systems | File audio | Ensoniq VFX-SD File | Chicken Systems Translator | Chicken Systems |
| .ELIST | Diekollegen.at | File audio | Emperor Media Player Playlist File | Emperor Media Player | Diekollegen.at |
| .EMB | Everest Software | File audio | Everest Embedded Bank File | Awave Studio | FMJ-Software |
| .EMD | FMJ-Software | File audio | ABT Extended Module File | Awave Studio | FMJ-Software |
| .EMP | Open Source | File audio | eMusic Music Download File | eMusic/J | Open Source |
| .EMX | Open Source | File audio | eMusic Download File | eMusic/J | Open Source |
| .EMY | Sony | File audio | eMelody Ringtone File | Sony Ericsson Mobile Phones | Sony Mobile Communications |
| .ENF | Thomson Reuters Corporation | File audio | EndNote Filter File | EndNote | Thomson Reuters Corporation |
| .ENS | Thomson Reuters Corporation | File audio | EndNote Style File | EndNote | Thomson Reuters Corporation |
| .EOP | Everyone Piano Inc. | File audio | EveryonePiano Music Score File | Everyone Piano | Everyone Piano Inc. |
| .EOV | Epson US | File audio | Epson Voice Creation Tool Voice Data | Voice Creation Tool | Epson US |
| .EPM | THE BAKED BEAN COMPANY | File audio | Electric Pipes Music | Electric Pipes | THE BAKED BEAN COMPANY |
| .EPT | Adobe Systems Incorporated | File audio | Adobe Encapsulated PostScript Interchange Format With TIFF Preview | Adobe Acrobat DC | Adobe Systems Incorporated |
| .ERB | Open Source | File audio | Ruby ERB Script | Microsoft Notepad | Microsoft Corporation |
| .ES | Interscape | File audio | ECHOSPEECH File | ECHOSPEECH | Interscape |
| .ESPS | Various Developers | File audio | ESPS Sampled Data File | The Hidden Markov Model Toolkit (HTK) | Machine Intelligence Laboratory |
| .ESU | Electronic Solutions Ulm | File audio | LokSound Electronic Sound File | LokSound | Electronic Solutions Ulm |
| .ETA | Google | File audio | Google Earth Placemark File | ESRI ArcGIS for Desktop | ESRI (Environmental Systems Research Institute) |
| .ETM | Etreppid Technologies, LLC | File audio | eTreppid Audio File | Unknown Software | Unknown Developer |
| .ETV | Open Source | File audio | eTreppid Audio Data | eTplayer | Open Source |
| .ETW | Enter Tech | File audio | Enter Tech Recorded Audio | Unknown Software | Unknown Developer |
| .EUPH | Nokia | File audio | Nokia File | Nokia phones | Nokia |
| .EVO | eZuce SRN | File audio | SeeVogh Player Video Recording | SeeVogh Player | eZuce SRN |
| .EVR | Qualcomm | File audio | Enhanced Variable Rate Audio File | RealPlayer | Realtime Technologies |
| .EXB | CAXA | File audio | CAXA Draft | IronCAD | IronCAD, LLC |
| .EXL | SysTools Solutions | File audio | Export Lister File | SysTools Export Notes | SysTools Solutions |
| .EXS | Apple | File audio | EXS Instrument | Apple Logic Pro | Apple |
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